Article
Stage and species specificity of antigens encoded by two geographic strains of Schistosoma Mansoni mRNA
Registro en:
BLANTON, Ronald Edward et al. Stage and species specificity of antigens encoded by two geographic strains of Schistosoma Mansoni mRNA. Journal of Parasitology, v. 72, n. 3, p. 445-453, 1986.
0022-3395
10.2307/3281685
Autor
Blanton, Ronald Edward
Horowitz, Sarah
Reis, Mitermayer Galvão dos
Rottman, Fritz
Andrade, Zilton de Araújo
Mahmoud, Adel A. F.
Resumen
Edna McConnell Clark Foundation, Al 15351 from the National Institute of Allergy and Infectious Diseases, and the National Foundation
for Infectious Diseases. Proteins translated in vitro from Schistosoma mansoni adult worm mRNA were assessed for their antigenic specificities compared to different stages, strains and species of the parasite. RNA was extracted from
both Puerto Rican and Brazilian parasites and directed the synthesis of high molecular weight proteins. Preabsorption of immune human serum with schistosomula was used to determine whether the in vitro translated
proteins contained antigens shared between the adult and this immature stage. Three antigens (Mr 36,000, 29,000, 18,000) were observed to be present in both stages. When adult worm mRNA from 2 different geographic strains of S. mansoni (Puerto Rican and Brazilian) were compared, certain antigenic differences were found in their in vitro translation products (proteins at Mr 78,000, 26,000, 24,000, 22,000, 15,500), suggesting that different antigenic pools may exist in nature. The species specificity of the in vitro proteins was assessed using individual sera from humans whose species of schistosome infection and egg counts were known. Immunoprecipitation with these sera demonstrated that a large number of immunologically cross-reactive proteins were sharedb etweenS . mansonia nd Schistosomah aematobiumb ut not Schistosomaj aponicum.A ntigenso r antigen complexes at Mr 47,000 and 37,000 were detected only in the immunoprecipitations using anti-S. mansoni sera, whereas an antigen of Mr 39,000 was precipitated only by anti-S. haematobium sera. The recognition of any 1 antigen or group of antigens, however, did not distinguish between intensities of infection.
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