Article
Identification of novel recombinants of hepatitis B virus genotypes F and G in human immunodeficiency virus-positive patients from Argentina and Brazil
Registro en:
ARAUJO, Natalia M.; et al. Identification of novel recombinants of hepatitis B virus genotypes F and G in human immunodeficiency virus-positive patients from Argentina and Brazil. Journal of General Virology, v. 94, p.150–158, 2013.
0022-1317
10.1099/vir.0.047324-0
Autor
Araujo, Natalia M.
Araujo, Oscar C.
Silva, Edinete M.
Nogueira, Cristiane A. Villela
Nabuco, Letícia C.
Parana, Raymundo
Bessone, Fernando
Gomes, Selma A.
Tiepo, Christian
Kay, Alan
Resumen
Hepatitis B virus (HBV) genotype G (HBV/G) infection is almost always detected along with a coinfecting
HBV strain that can supply HBeAg, typically HBV/A2. In this study we describe, in two
human immunodeficiency virus (HIV)-positive patients from Argentina and Brazil, the first report of
HBV/G infection in Argentina and co-circulation of HBV/G, HBV/F and G/F recombinants in the
American continent. HBV isolates carrying the 36 bp insertion of HBV/G were the most prevalent
in both patients, with .99% of colonies hybridizing to a probe specific for this insertion.
Phylogenetic analyses of full-length genomes and precore/core fragments revealed that F4 and
F1b were the co-infecting subgenotypes in the Brazilian and Argentinian patients, respectively.
Bootscanning analysis provided evidence of recombination in several clones from both patients,
with recombination breakpoints located mainly at the precore/core region. These data should
encourage further investigations on the clinical implications of HBV/G recombinants in HBV/HIV
co-infected patients.