Article
Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae
Registro en:
ROCHA, A. et al. Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae. Memórias do Instituto Oswaldo Cruz, v. 104, n. 4, p. 621–625, jul. 2009.
1678-8060
10.1590/S0074-02762009000400015
Autor
Rocha, Abraham
Braga, Cynthia
Belém, Marcela
Carrera, Arturo
Aguiar-Santos, Ana
Oliveira, Paula
Texeira, Maria José
Furtado, André
Resumen
FIOCRUZ / FUNASA (projetos 268-369-433, sub-projeto 17), FIOTEC / SVS (projetos 573-17 e 801-17), Departamento de Parasitologia do Centro de Pesquisas Aggeu Magalhães Significant advances were made in the diagnosis of filariasis in the 1990s with the emergence of three new alternative tools: ultrasound and tests to detect circulating antigen using two monoclonal antibodies, Og4C3 and AD12-ICT-card. This study aimed to identify which of these methods is the most sensitive for diagnosis of infection. A total of 256 individuals, all male and carrying microfilariae (1-15,679 MF/mL), diagnosed by nocturnal venous blood samples, were tested by all three techniques. The tests for circulating filarial antigen concurred 100% and correctly identified 246/256 (96.69%) of the positive individuals, while ultrasound detected only 186/256 (73.44%). Of the circulating antigen tests, ICT-card was the most convenient method for identification of Wuchereria bancrofti carriers. It was easy to perform, practical and quick.