Na/K-ATPase assay in the intact mice lung subjected to perfusion

dc.creatorAlbuquerque, Cassiano Felippe Gonçalves de
dc.creatorBurth, Patrícia
dc.creatorSilva, Adriana Ribeiro
dc.creatorMoraes, Isabel Matos Medeiros de
dc.creatorOliveira, Flora Magno de Jesus
dc.creatorSantelli, Ricardo Erthal
dc.creatorFreire, Aline Soares
dc.creatorIbrahim, Mauricio Younes
dc.creatorFaria Neto, Hugo Caire de Castro
dc.creatorFaria, Mauro Velho de Castro
dc.date2015-05-27T13:39:50Z
dc.date2015-05-27T13:39:50Z
dc.date2014
dc.date.accessioned2023-09-27T00:01:29Z
dc.date.available2023-09-27T00:01:29Z
dc.identifierALBUQUERQUE, Cassiano Felippe G. et al. Na/K-ATPase assay in the intact mice lung subjected to perfusion. BMC Research Notes, v.7:798, 6p, 2014.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/10544
dc.identifier10.1186/1756-0500-7-798
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8896648
dc.descriptionAmong the characteristics of acute respiratory distress syndrome (ARDS) is edema formation and its resolution depends on pneumocyte Na/K-ATPase activity. Increased concentration of oleic acid (OA) in plasma induces lung injury by targeting Na/K-ATPase and, thus, interfering in sodium transport. FINDINGS: Presently, we adapted a radioactivity-free assay to detect Na/K-ATPase activity in perfused lung mice, comparing the inhibitory effect of ouabain and OA. We managed to perfuse only the lung, avoiding the systemic loss of rubidium. Rb+ incorporation into lung was measured by inductively coupled plasma optical emission spectrometry (ICP OES) technique, after lung tissue digestion. Na/K-ATPase activity was the difference between Rb+ incorporation with or without ouabain. Lung Na/K-ATPase was completely inhibited by perfusion with ouabain. However, OA caused a partial inhibition. CONCLUSIONS: In the present work the amount of incorporated Rb+ was greater than seen in our previous report, showing that the present technique is trustworthy. This new proposed assay may allow researchers to study the importance of Na/K-ATPase activity in lung pathophysiology.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBioMed Central
dc.rightsopen access
dc.subjectPulmão
dc.subjectLung
dc.subjectNa/K-ATPase
dc.subjectInductively coupled plasma optical emission spectrometer
dc.subjectAcute respiratory distress syndrome (ARDS)
dc.subjectpneumocyte Na/K-ATPase
dc.titleNa/K-ATPase assay in the intact mice lung subjected to perfusion
dc.typeArticle


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