Article
Na/K-ATPase assay in the intact mice lung subjected to perfusion
Registro en:
ALBUQUERQUE, Cassiano Felippe G. et al. Na/K-ATPase assay in the intact mice lung subjected to perfusion. BMC Research Notes, v.7:798, 6p, 2014.
10.1186/1756-0500-7-798
Autor
Albuquerque, Cassiano Felippe Gonçalves de
Burth, Patrícia
Silva, Adriana Ribeiro
Moraes, Isabel Matos Medeiros de
Oliveira, Flora Magno de Jesus
Santelli, Ricardo Erthal
Freire, Aline Soares
Ibrahim, Mauricio Younes
Faria Neto, Hugo Caire de Castro
Faria, Mauro Velho de Castro
Resumen
Among the characteristics of acute respiratory distress syndrome (ARDS) is edema formation and its resolution depends on pneumocyte Na/K-ATPase activity. Increased concentration of oleic acid (OA) in plasma induces lung injury by targeting Na/K-ATPase and, thus, interfering in sodium transport.
FINDINGS:
Presently, we adapted a radioactivity-free assay to detect Na/K-ATPase activity in perfused lung mice, comparing the inhibitory effect of ouabain and OA. We managed to perfuse only the lung, avoiding the systemic loss of rubidium. Rb+ incorporation into lung was measured by inductively coupled plasma optical emission spectrometry (ICP OES) technique, after lung tissue digestion. Na/K-ATPase activity was the difference between Rb+ incorporation with or without ouabain. Lung Na/K-ATPase was completely inhibited by perfusion with ouabain. However, OA caused a partial inhibition.
CONCLUSIONS:
In the present work the amount of incorporated Rb+ was greater than seen in our previous report, showing that the present technique is trustworthy. This new proposed assay may allow researchers to study the importance of Na/K-ATPase activity in lung pathophysiology.