Otro
Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis
Registro en:
Memorias do Instituto Oswaldo Cruz, v. 100, n. 4, p. 427-429, 2005.
0074-0276
10.1590/S0074-02762005000400014
S0074-02762005000400014
2-s2.0-23944497098.pdf
2-s2.0-23944497098
Autor
Barcelos, Ivanildes Solange da Costa
Ferreira, Marcelo Simão
Moura, Leandro Pajuaba de
Biondi, Germano Francisco
Costa-Cruz, Julia Maria
Resumen
Paired samples of cerebrospinal fluid (CSF) and serum of 30 patients - 10 with active, 10 with inactive neurocysticercosis (NCC), and 10 control subjects - were evaluated by enzyme-linked immunosorbent assay (ELISA) using two Taenia crassiceps metacestode extracts as antigen in order to detect IgG antibodies. In active NCC, high levels of IgG were detected (p < 0.05). The CSF samples showed 80% (CI 72-88) of reactivity in the saline extract (S) and 90% (CI 84-95) in sodium dodecyl sulphate (SDS) and the serum samples were reactive in 90% (CI 84-95) and 100% (CI 98-100) in the S and SDS antigenic extracts, respectively. The use of the paired samples of CSF and serum in active NCC showed equivalent results suggesting that the serum samples could be used as a screening in those patients whose CSF puncture is counter-indicated.