Preprint
High-resolution genome-wide analysis is essential for the identification of ambiguous Aeromonas strains
Registro en:
MELO, Beatriz Souza Toscano de et al. High-resolution genome-wide analysis is essential for the identification of ambiguous Aeromonas strains. FEMS Microbiology Letters, p. 1-13, Dec. 2019.
0378-1097
10.1093/femsle/fnz245
1574-6968
Autor
Melo, Beatriz Souza Toscano de
Mendes-Marques, Carina Lucena
Campos, Túlio de Lima
Almeida, Alzira Maria Paiva de
Leal, Nilma Cintra
Xavier, Danilo Elias
Resumen
Aeromonads are mainly opportunistic pathogens; however, many species are emerging as important human pathogens. Therefore, monitoring these bacteria and their accurate characterization of its species is highly important. Aeromonas Aer593 strain was recovered from a diarrhoea outbreak and did not group with any previously described Aeromonas species by housekeeping gene sequencing. To clarify the taxonomic position of Aer593, its genome was sequenced and analysed by multilocus phylogenetic analysis (MLPA), in silico DNA-DNA hybridization (isDDH), average nucleotide identity (ANI) and core genome-based phylogenetic analyses. The MLPA with the housekeeping genes gyrB, rpoD, recA, dnaJ, gyrA, and dnaX ranked the Aer593 isolate into an independent branch suggesting that it could represent a new species. However, the identity percentages of Aer593 to A. caviae strains using robust genomic analysis by isDDH and ANI were at least 81.3% and 97.8%, respectively, defining Aer593 as A. caviae. Multilocus sequence typing (MLST) presented an exact match against only a single allele (groL96) and the novel ST648 was assigned for this strain. The core genome-based phylogenetic analyses with a total of 863 orthologous genes also grouped the Aer593 isolate with A. caviae reference strains. These findings warn about the possibility of misidentification of some Aeromonas strains by MLPA and show that high-resolution genome-wide analysis is essential for the correct identification of ambiguous Aeromonas strains. 2050-01-01