A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni

dc.creatorAdema, Coen M.
dc.creatorLuo, Mei-Zhong
dc.creatorHanelt, Ben
dc.creatorHertel, Lynn A.
dc.creatorMarshall, Jennifer J.
dc.creatorSi-Ming, Zhang
dc.creatorDeJong, Randall J.
dc.creatorKim, Hye-Ran
dc.creatorKudrna, David
dc.creatorWing, Rod A.
dc.creatorSoderlund, Cari
dc.creatorKnight, Matty
dc.creatorLewis, Fred A.
dc.creatorCaldeira, Roberta Lima
dc.creatorPassos, Liana Konovaloff Jannotti
dc.creatorCarvalho, Omar dos Santos
dc.creatorLoker, Eric S.
dc.date2013-05-14T13:50:34Z
dc.date2013-05-14T13:50:34Z
dc.date2006
dc.date.accessioned2023-09-26T23:56:02Z
dc.date.available2023-09-26T23:56:02Z
dc.identifierADEMA, Coen Martinus et al. A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, Suppl. I, p. 167-177, 2006.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/6489
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895743
dc.descriptionTo provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research Institute (NHGRI), produced a high quality bacterial artificial chromosome (BAC) library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil) that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library(BG_BBa) consists of 61824 clones (136.3 kb average insert size) and provides 9.05 × coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clonesindicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads,299860 nt) indicated that the genome of B. glabrata contains ~ 63% AT, and disclosed several novel genes, transposableelements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI atcost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectGenomics
dc.subjectGene discovery
dc.subjectFingerprinting
dc.subjectSchistosomiasis
dc.subjectMedical malacology
dc.titleA bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni
dc.typeArticle


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