Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library

dc.creatorGalvão, Cleber Eduardo
dc.creatorFragoso, Stenio Perdigão
dc.creatorOliveira, Carina Elisei de
dc.creatorForner, Odinéia
dc.creatorPereira, Renata Ribeiro Bastos
dc.creatorSoares, Cleber Oliveira
dc.creatorRosinha, Grácia Maria Soares
dc.date2018-09-12T15:14:52Z
dc.date2018-09-12T15:14:52Z
dc.date2017
dc.date.accessioned2023-09-26T23:54:13Z
dc.date.available2023-09-26T23:54:13Z
dc.identifierGALVÃO, Cleber Eduardo et al. Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. BMC Microbiology, v. 17, n. 202, 2017.
dc.identifier1471-2180
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28690
dc.identifier10.1186/s12866-017-1110-7
dc.identifier1471-2180
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895444
dc.descriptionCaseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. For this, a wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli. For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. This way, concluded that, proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes.
dc.formatapplication/pdf
dc.languagepor
dc.publisherSpringer Nature
dc.rightsopen access
dc.subjectCorynebacterium pseudotuberculosis
dc.subjectLymphadenitis
dc.subjectGene Expression
dc.subjectGene Library
dc.subjectVaccines
dc.subjectLinfadenitis
dc.subjectExpresión Génica
dc.subjectBiblioteca de Genes
dc.subjectVacunas de ADN
dc.subjectLinfadenite
dc.subjectExpressão Gênica
dc.subjectBiblioteca Gênica
dc.subjectVacinas de DNA
dc.titleIdentification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library
dc.typeArticle


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