Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus

dc.creatorSoares, Alexandra M. S.
dc.creatorOliveira, Jose T. A.
dc.creatorRocha, Cláudia Q.
dc.creatorFerreira, André T. S.
dc.creatorPerales, Jonas
dc.creatorZanatta, Ana Caroline
dc.creatorVilegas, Wagner
dc.creatorSilva, Carolina R.
dc.creatorCosta Junior, Livio M.
dc.date2019-02-12T12:18:15Z
dc.date2019-02-12T12:18:15Z
dc.date2018
dc.date.accessioned2023-09-26T23:49:21Z
dc.date.available2023-09-26T23:49:21Z
dc.identifierSOARES, Alexandra M. S. et al. Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus. Plos One, v.13, n.7, e0200848, 12p, July 2018.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31563
dc.identifier10.1371/journal.pone.0200848
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894587
dc.descriptionSeed exudates are plant-derived natural bioactive compounds consisting of a complex mixture of organic and inorganic molecules. Plant seed exudates have been poorly studied against parasite nematodes. This study was undertaken to identify proteins in the Myracrodruon urundeuva seed exudates and to assess the anthelmintic activity against Haemonchus contortus, an important parasite of small ruminants. M. urundeuva seed exudates (SEX) was obtained after immersion of seeds in sodium acetate buffer. SEX was fractionated with ammonium sulfate at 0-90% concentration to generate the ressuspended pellet (SEXF1) and the supernatant (SEXF2). SEX, SEXF1, and SEXF2 were exhaustively dialyzed against distilled water (cut-off: 12 kDa) and the protein contents determined. Mass spectrometry analyses of SEX, SEXF1, and SEXF2 were done to identify proteins and secondary metabolites. The seed exudates contained protease, protease inhibitor, peptidase, chitinase, and lipases as well as the low molecular weight secondary compounds ellagic acid and quercetin rhamnoside. SEX inhibited H. contortus larval development (LDA) (IC50 = 0.29 mg mL-1), but did not affect larval exsheathment (LEIA). On the other hand, although SEXF1 and SEXF2 inhibited H. contortus LEIA (IC50 = 1.04 and 0.93 mg mL-1, respectively), they showed even greater inhibition efficiency of H. contortus larval development (IC50 = 0.29 and 0.42 mg mL-1, respectively). To the best of our knowledge, this study is the first to show the anthelmintic activity of plant exudates against a gastrointestinal nematode. Moreover, it suggests the potential of exuded proteins as candidates to negatively interfere with H. contortus life cycle.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectMyracrodruon urundeuva
dc.subjectProteoma
dc.subjectAtividade anti-helmíntica
dc.subjectHaemonchus contortus
dc.subjectSementes
dc.subjectMyracrodruon urundeuva
dc.subjectProteome
dc.subjectAnthelmintic activity
dc.subjectHaemonchus contortus
dc.subjectSeeds
dc.titleMyracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus
dc.typeArticle


Este ítem pertenece a la siguiente institución