Article
Protease expression analysis in recently field-isolated strains of Trypanosoma cruzi: a heterogeneous profile of cysteine protease activities between TC I and TC II major phylogenetic groups
Registro en:
FAMPA, P. et al. Protease expression analysis in recently field-isolated strains of Trypanosoma cruzi: a heterogeneous profile of cysteine protease activities between TC I and TC II major phylogenetic groups. Parasitology, v. 135, p. 1093–1100, 2008.
0031-1820
10.1017/S0031182008004587
1469-8161
Autor
Fampa, P.
Lisboa, C. V.
Jansen, Ana Maria
Santos, A. L. S.
Ramirez, M. I.
Resumen
Protease expression among TCI and TCII field isolates was analysed. Gelatin-containing gels revealed hydrolysis bands
with molecular masses ranging from 45 to 66 kDa. The general protease expression profile showed that TCII isolates
presented higher heterogeneity compared to TCI. By utilizing protease inhibitors, we showed that all active proteases at
acid pH are cysteine-proteases and all proteases active at alkaline pH are metalloproteases. However, the expression of
cruzipain, the T. cruzi major cysteine-protease, did not reproduce a heterogeneous TCII cysteine zymogram profile.
Dendogram analyses based on presence/absence matrices of proteases and cruzipain bands showed a TCI separation from
the TCII group with 50–60% similarity. We suggest that the observed cysteine protease diversification contributes to
differential host infection between TCI and II genotypes. 2022-01-01