Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA

dc.creatorCuervo, Patrícia
dc.creatorCupolillo, Elisa
dc.creatorSegura, Iris
dc.creatorSaravia, Nancy
dc.creatorFernandes, Octavio
dc.date2020-10-26T18:56:13Z
dc.date2020-10-26T18:56:13Z
dc.date2002
dc.date.accessioned2023-09-26T23:46:18Z
dc.date.available2023-09-26T23:46:18Z
dc.identifierCUERVO, Patricia et al. Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 6, p. 877-880, Sept. 2002.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44209
dc.identifier10.1590/S0074-02762002000600023
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894035
dc.descriptionAmerican trypanosomiasis is a common zoonosis in Colombia and Trypanosoma cruzi presents a wide distribution throughout the country. Although some studies based on enzyme electrophoresis profiles have described the population structure of the parasite, very few molecular analyses of genotipic markers have been conducted using Colombian strains. In this study, we amplified the non-transcribed spacer of the mini-gene by PCR, typing the isolates as T. cruzi I, T. cruzi zymodeme 3 or T. rangeli. In addition, the internal transcribed spacers of the ribosomal gene concomitant with the 5.8S rDNA were amplified and submitted to restriction fragment polymorphism analysis. The profiles were analyzed by a numerical methodology generating a phenetic dendrogram that shows heterogeneity among the T. cruzi isolates. This finding suggests a relationship between the complexity of the sylvatic transmission cycle in Colombia and the diversity of the sylvan parasites.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectEspaçadores transcritos internos
dc.subjectMini-exon gene
dc.subjectDNA ribossômico
dc.subjectColômbia
dc.subjectTrypanosoma cruzi
dc.subjectInternal transcribed spacers
dc.subjectMini-exon gene
dc.subjectRibosomal DNA
dc.subjectColombia
dc.titleGenetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA
dc.typeArticle


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