Article
Molecular Serogrouping of Listeria monocytogenes from Brazil Using PCR
Registro en:
CAMARGO, Anderson Carlos; et al. Molecular Serogrouping of Listeria monocytogenes from Brazil Using PCR. Journal of Food Protection, v.79, n.1, p.144–147, 2016.
0362-028X
1944-9097
Autor
Camargo, Anderson Carlos
Vallim, Deyse Christina
Hofer, Eernesto
Nero, Luís Augusto
Resumen
We assessed the serotype distribution of Listeria monocytogenes isolates from clinical, beef, and environment samples using
two PCR-based protocols for serogrouping. A panel of 134 isolates (22 clinical samples, 79 samples of beef cuts, and 33 samples
from the beef processing environment) were subjected to conventional serology and identified as serotypes 1/2a (n ¼ 12), 1/2b (n
¼ 21), 1/2c (n ¼ 71), and 4b (n ¼ 30). Isolates from clinical samples were predominantly serotype 4b, and the most prevalent
serotype among the beef cut and environment samples was 1/2c. The protocol described by M. Doumith, C. Buchrieser, P.
Glaser, C. Jacquet, and P. Martin (J. Clin. Microbiol. 42:3819–3822, 2004) produced contradictory results for seven 1/2a isolates,
which were positive for lmo1118 and had the profile IIc (serotypes 1/2c and 3c). Fifteen serotype 4b isolates amplified the target
lmo0737, with the atypical profile IVb variant 1. The results obtained with the protocol described by M. K. Borucki and D. R.
Call (J. Clin. Microbiol. 41:5537–5540, 2003) were in full agreement with those of the conventional serology. We recommend
using this multiplex PCR approach by adding one pair of the reported primers to the panel to reduce total effort by one PCR while
maintaining specificity. We present additional recommendations to improve the efficiency and reproducibility of this
serogrouping assay. 2030-01-01