Isolation of natural inhibitors of papain obtained from Carica papaya latex

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMonti, Rubens
dc.creatorContiero, Jonas
dc.creatorGoulart, Antonio José
dc.date2014-05-27T11:21:12Z
dc.date2016-10-25T18:20:09Z
dc.date2014-05-27T11:21:12Z
dc.date2016-10-25T18:20:09Z
dc.date2004-12-01
dc.date.accessioned2017-04-06T01:11:13Z
dc.date.available2017-04-06T01:11:13Z
dc.identifierBrazilian Archives of Biology and Technology, v. 47, n. 5, p. 747-754, 2004.
dc.identifier1516-8913
dc.identifierhttp://hdl.handle.net/11449/67993
dc.identifierhttp://acervodigital.unesp.br/handle/11449/67993
dc.identifier10.1590/S1516-89132004000500010
dc.identifierS1516-89132004000500010
dc.identifierWOS:000225349100010
dc.identifier2-s2.0-19944371762.pdf
dc.identifier2-s2.0-19944371762
dc.identifierhttp://dx.doi.org/10.1590/S1516-89132004000500010
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889377
dc.descriptionStudies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P -III).
dc.languageeng
dc.relationBrazilian Archives of Biology and Technology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectEsterase activity
dc.subjectNatural papain inhibitor
dc.subjectProtease inhibitor
dc.titleIsolation of natural inhibitors of papain obtained from Carica papaya latex
dc.typeOtro


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