Development of quantitative PCR and digital PCR for the quantification of Leishmania infantum in dogs

dc.creatorPereira, Diego Carlos Andrade
dc.creatorTeixeira Neto, Rafael Gonçalves
dc.creatorLopes, Valeriana Valadares
dc.creatorPena, Héber Paulino
dc.creatorPaz, Gustavo Fontes
dc.creatorCustodio, Carlos Henrique Xavier
dc.creatorBelo, Vinícius Silva
dc.creatorFonseca Júnior, Antônio Augusto da
dc.creatorSilva, Eduardo Sérgio da
dc.date2023-03-15T19:08:32Z
dc.date2023-03-15T19:08:32Z
dc.date2023
dc.date.accessioned2023-09-26T23:43:53Z
dc.date.available2023-09-26T23:43:53Z
dc.identifierPEREIRA, Diego Carlos Andrade et al. Development of quantitative PCR and digital PCR for the quantification of Leishmania infantum in dogs. Mol Cell Biochem, 2023. doi: 10.1007/s11010-023-04672-9.
dc.identifier0300-8177
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/57393
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8893618
dc.descriptionLeishmaniasis is a zoonotic disease with worldwide distribution. In the Americas, the causative agent of the visceral form is the protozoa Leishmania (Leishmania) infantum. Transmission to the host or vertebrate reservoir occurs through the bite of infected arthropod females like Lutzomyia longipalpis. The epidemiological connection between the infection in dogs and humans generate constant studies about the relationship between the parasite and the canine host, including the development of methods and tests for the detection and quantification ofLeishmania (L.) infantum. Both conventional PCR (cPCR) and quantitative PCR (qPCR) can be used in the diagnosis of the parasite. Dropet Digital PCR (ddPCR) is another useful tool. Knowing the parasite load and its relationship with the clinical signs of naturally infected dogs is useful in research development and for establishing treatments that reduce the transmission of the disease. In this study, thirty-nine clinical samples of spleen from dogs naturaly infected by L. infantum were collected after necropsy. Two molecular tools were used to quantify the parasite load (qPCR and ddPCR) and there was 100% agreement in the results of the them. The tools developed in this work are important for the detection of L. infantum in dogs and humans. Droplet Digital PCR does not require a standard curve and is easy to standardize. In such manner, this new tool can generate more in-depth information in the broad debate about parasitic loads and the pathogenesis of leishmaniasis.
dc.description2099-12-31
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer
dc.rightsrestricted access
dc.subjectDroplet digital PCR
dc.subjectLeishmaniasis
dc.subjectMolecular diagnosis
dc.subjectParasite load
dc.subjectddPCR
dc.titleDevelopment of quantitative PCR and digital PCR for the quantification of Leishmania infantum in dogs
dc.typeArticle


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