In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBuffa Filho, Waldemar
dc.creatorBolzani, Vanderlan da Silva
dc.creatorFurlan, Maysa
dc.creatorVaz Pereira, Sarazete Izidia
dc.creatorPereira, Ana Maria Soares
dc.creatorFrançab, Suzelei Castro
dc.date2014-05-27T11:21:09Z
dc.date2016-10-25T18:19:54Z
dc.date2014-05-27T11:21:09Z
dc.date2016-10-25T18:19:54Z
dc.date2004-09-19
dc.date.accessioned2017-04-06T01:10:22Z
dc.date.available2017-04-06T01:10:22Z
dc.identifierArkivoc, v. 2004, n. 6, p. 137-146, 2004.
dc.identifier1424-6376
dc.identifierhttp://hdl.handle.net/11449/67879
dc.identifierhttp://acervodigital.unesp.br/handle/11449/67879
dc.identifier10.3998/ark.5550190.0005.617
dc.identifierWOS:000224153100017
dc.identifierWOS000224153100017.pdf
dc.identifier2-s2.0-5444226445
dc.identifierhttp://www.arkat-usa.org/get-file/19210/
dc.identifierhttp://dx.doi.org/10.3998/ark.5550190.0005.617
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889280
dc.descriptionCell culture of Maytenus ilicifolia were established in order to produce and to quantify the antitumoral and antioxidant quinonemethide triterpenes. In vitro calli were induced from leaf explants of native plants and cultured in semi-solid medium under controlled conditions of humidity, temperature and photoperiod. The quinonemethide triterpenes showed maximum accumulation in the logarithmic phase growth of the cell culture. A rapid, sensitive and reliable reverse-phase HPLC method was used for quantitative determination of the antitumoral and antioxidant quinonemethide triterpenes, 22β-hydroxymaytenin and maytenin in callus of Maytenus ilicifolia. Well resolved peaks with good detection response and linearity in the range 1.0 - 100 μg/mL were obtained. This quantitative work was performed by an external standard method.
dc.languageeng
dc.relationArkivoc
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectin vitro system, Celastraceae
dc.subjectMaytenus ilicifolia
dc.subjectQuantitative determination
dc.subjectQuinonemethide triterpenes, Reverse-phase high-performance liquid chromatography
dc.subject22beta hydroxymaytenin
dc.subjectantineoplastic agent
dc.subjectantioxidant
dc.subjectmaytenin
dc.subjectmaytenus ilicifolia extract
dc.subjectplant extract
dc.subjectquinone derivative
dc.subjecttriterpene derivative
dc.subjectunclassified drug
dc.subjectbioaccumulation
dc.subjectCelastraceae
dc.subjectcell culture
dc.subjectdrug potency
dc.subjectdrug synthesis
dc.subjectexplant
dc.subjecthumidity
dc.subjectin vitro study
dc.subjectMaytenus
dc.subjectphotosynthesis
dc.subjectplant cell
dc.subjectplant leaf
dc.subjectquantitative analysis
dc.subjectreliability
dc.subjectreversed phase high performance liquid chromatography
dc.subjectstandard
dc.subjecttechnique
dc.subjecttemperature dependence
dc.titleIn vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography
dc.typeOtro


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