Article
Assessment of serological tests for the diagnosis of canine visceral leishmaniasis
Registro en:
SILVA, Denise Amaro da et al. Assessment of serological tests for the diagnosis of canine visceral leishmaniasis. Veterinary Journal, v. 195, p. 252-253, 2013.
1090-0233
10.1016/j.tvjl.2012.06.010
1532-2971
Autor
Silva, Denise Amaro da
Madeira, Maria de Fátima
Abrantes, Tuanne Rotti
Barbosa Filho, Carlos José de Lima
Figueiredo, Fabiano Borges
Resumen
An immunoenzymatic assay (ELISA), an indirect immunofluorescence antibody test (IFAT) with different antigens (ELISA-Leishmania chagasi, ELISA-L. major-like, IFAT-L. chagasi and IFAT-L. major-like), and an immunochromatographic test were assessed for the diagnosis of canine visceral leishmaniasis (CVL). Serum samples from 144 dogs from an endemic area for visceral leishmaniasis in the municipality of Rio de Janeiro were tested. The sensitivities of the serological tests were 93%, 100%, 73%, 60% and 93%, with specificities of 87%, 92%, 77%, 96% and 92% for the ELISA-L. major-like, ELISA-L. chagasi, IFAT-L. major-like, IFAT-L. chagasi and the immuno chromatographic test, respectively. ELISA-L. chagasi was the best test for the diagnosis of CVL, but the immunochromatographic test could be a useful alternative as it offers simple and rapid diagnosis without the need for a specialized laboratory. 2020-09-26