Otro
Lack of genotoxicity of formocresol, paramonochlorophenol, and calcium hydroxide on mammalian cells by comet assay
Registro en:
Journal of Endodontics, v. 30, n. 8, p. 593-596, 2004.
0099-2399
10.1097/01.DON.0000121614.10075.A3
WOS:000222923700008
2-s2.0-4143135152
Autor
Ribeiro, Daniel Araki
Marques, Mariângela Esther Alencar
Salvadori, Daisy Maria Favero
Resumen
Formocresol, paramonochlorophenol, and calcium hydroxide are widely used in dentistry because of their antibacterial activities in root canal disinfection. However, the results of genotoxicity studies using these materials are inconsistent in literature. The goal of this study was to examine the genotoxic potential of formocresol, paramonochlorophenol, and calcium hydroxide using mouse lymphoma cells and human fibroblasts cells in vitro by the comet assay. Data were assessed by Kruskal-Wallis nonparametric test. The results showed that all compounds tested did not cause DNA damage for the tail moment or tail intensity parameters. These findings suggest that formocresol, paramonochlorophenol, and calcium hydroxide do not promote DNA damage in mammalian cells and that the comet assay is a suitable tool to investigate genotoxicity.