Inhibition of ecto-ATPase activities impairs HIV-1 infection of macrophages

dc.creatorSchachter, Julieta
dc.creatorDelgado, Kelly Valcárcel
dc.creatorSouza, Victor Barreto de
dc.creatorBou-Habib, Dumith Chequer
dc.creatorPersechini, Pedro Muanis
dc.creatorFernandes, José Roberto Meyer
dc.date2015-10-27T14:12:21Z
dc.date2016-05-01T07:30:06Z
dc.date2015
dc.date.accessioned2023-09-26T23:31:49Z
dc.date.available2023-09-26T23:31:49Z
dc.identifierSCHACHTER, Julieta; et al. nhibition of ecto-ATPase activities impairs HIV-1 infection of macrophages. Immunibiology, v.220, n.5, p.589-596, May 2015.
dc.identifier0171-2985
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/12094
dc.identifier10.1016/j.imbio.2014.12.004
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891555
dc.descriptiontNucleotides and nucleosides are secreted into extracellular media at different concentrations as a conse-quence of different physiologic and pathological conditions. Ecto-nucleotidases, enzymes present on thesurface of most cells, hydrolyze these extracellular nucleotides and reduce the concentration of them,thus affecting the activation of different nucleotide and nucleoside receptors. Also, ecto-nucleotidasesare present in a number of microorganisms and play important roles in host–pathogen interactions.Here, we characterized the ecto-ATPase activities present on the surface of HIV-1 particle and humanmacrophages as well. We found that the kinetic properties of HIV-1 and macrophage ecto-ATPases aresimilar, suggesting that the enzyme is the same. This ecto-ATPase activity was increased in macrophagesinfected in vitro with HIV-1. Using three different non-related ecto-ATPase inhibitors—POM-1, ARL67156and BG0—we showed that the inhibition of these macrophage and viral ecto-ATPase activities impairsHIV-1 infection. In addition, we also found that elevated extracellular concentrations of ATP inhibit HIV-1production by infected macrophages.
dc.description2016-04-30
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectEcto-ATPase
dc.subjectCD39
dc.subjectInfected macrophages
dc.subjectHIV Infection
dc.subjectInfecções por HIV
dc.subjectMacrófagos
dc.subjectNucleotídeos
dc.subjectNucleosídeos
dc.titleInhibition of ecto-ATPase activities impairs HIV-1 infection of macrophages
dc.typeArticle


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