Screening for pectinolytic activity of wood-rotting basidiomycetes and characterization of the enzymes

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorXavier-Santos, S.
dc.creatorCarvalho, C. C.
dc.creatorBonfá, M.
dc.creatorSilva, R.
dc.creatorCapelari, M.
dc.creatorGomes, E.
dc.date2014-05-27T11:21:04Z
dc.date2016-10-25T18:19:31Z
dc.date2014-05-27T11:21:04Z
dc.date2016-10-25T18:19:31Z
dc.date2004-04-20
dc.date.accessioned2017-04-06T01:09:02Z
dc.date.available2017-04-06T01:09:02Z
dc.identifierFolia Microbiologica, v. 49, n. 1, p. 46-52, 2004.
dc.identifier0015-5632
dc.identifierhttp://hdl.handle.net/11449/67709
dc.identifierhttp://acervodigital.unesp.br/handle/11449/67709
dc.identifier10.1007/BF02931645
dc.identifierWOS:000220630700009
dc.identifier2-s2.0-1842523301
dc.identifierhttp://dx.doi.org/10.1007/BF02931645
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889128
dc.descriptionSeventy-five fungal strains from different groups of basidiomycetes, newly isolated from rotten wood, were screened for pectinolytic activity. Despite the fact that basidiomycetes are scarcely referred to as pectinase producers, the polygalacturonase (PG) activity was detected in 76 % of the strains; 16 % with activity higher than 40 nkat/g, 40 % between 13.3 and 40 nkat/g, and 44 % with activity lower than 13.3 nkat/g. The highest productions were obtained among the fungi from order Aphyllophorales, family Polyporaceae. The characterization of the enzymes from the highest PG producers (Lentinus sp., Gloeophyllum striatum, Pycnoporus sanguineus, Schizophyllum commune) showed optimum temperature for catalytic activity at 60-70°C and two peaks of pH optimum (3.5-4.5 and 8.5-9.5). The enzymes exhibited high pH stability (3.0-11.0) but after incubation at 40°C for 1 h their activity dropped by 18-73 %.
dc.languageeng
dc.relationFolia Microbiologica
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectpectin
dc.subjectpolygalacturonase
dc.subjectBasidiomycetes
dc.subjectenzyme stability
dc.subjectenzymology
dc.subjectisolation and purification
dc.subjectLentinus
dc.subjectmetabolism
dc.subjectmicrobiology
dc.subjectpH
dc.subjectPolyporaceae
dc.subjecttemperature
dc.subjectwood
dc.subjectBasidiomycota
dc.subjectEnvironmental Microbiology
dc.subjectEnzyme Stability
dc.subjectHydrogen-Ion Concentration
dc.subjectLentinula
dc.subjectPectins
dc.subjectPolygalacturonase
dc.subjectSchizophyllum
dc.subjectTemperature
dc.subjectWood
dc.subjectAphyllophorales
dc.subjectFungi
dc.subjectGloeophyllum
dc.subjectPycnoporus sanguineus
dc.subjectSchizophyllum commune
dc.titleScreening for pectinolytic activity of wood-rotting basidiomycetes and characterization of the enzymes
dc.typeOtro


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