Article
Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
Registro en:
PAREDES, Bruno Diaz et al. Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies. Stem Cell Research, v. 38, p. 1-5, 2019.
1873-5061
10.1016/j.scr.2019.101454
Autor
Paredes, Bruno Diaz
Martins, Gabriele Louise Soares
Azevedo, Carine Machado
Sampaio, Gabriela Louise de Almeida
Nonaka, Carolina Kymie Vasques
Silva, Katia Nunes da
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Souza, Bruno Solano de Freitas
Resumen
CNPq, CAPES, FAPESB, Ministry of Health, National Induced Pluripotent Stem Cell Biobank and the National Institute of Science and Technology for Regenerative Medicine. Sickle cell disease (SCD) is one of the most prevalent and severe monogenetic disorders. Previously, we generated iPS cell lines from SCD patients. Here, we generated iPS cell lines from three age-, ethnicity- and gender-matched healthy individuals as control cell lines. Cell reprogramming was performed using erythroblasts expanded from PBMC by a non-integrative method. SCD-iPSC controls expressed pluripotency markers, presented a normal karyotype, were able to differentiate into the three germ layers in embryoid body spontaneous differentiation and confirmed to be integration-free. The cell lines generated here may be used as matched healthy controls for SCD studies.