The aquatic environment as a reservoir of Vibrio cholerae O1 in hydrographic basins of the state of Pernambuco, Brazil

dc.creatorMendes-Marques, Carina Lucena
dc.creatorSilveira Filho, Vladimir da Mota
dc.creatorCosta, Ana Paula Rocha da
dc.creatorNunes, Mariana de Lira
dc.creatorSilva Filho, Sandoval Vieira da
dc.creatorFigueirôa, Ângela Cristina Torres de Araújo
dc.creatorHofer, Ernesto
dc.creatorAlmeida, Alzira Maria Paiva de
dc.creatorLeal, Nilma Cintra
dc.date2017-12-14T12:19:49Z
dc.date2017-12-14T12:19:49Z
dc.date2013
dc.date.accessioned2023-09-26T23:26:41Z
dc.date.available2023-09-26T23:26:41Z
dc.identifierMENDES-MARQUES, Carina Lucena et al. The aquatic environment as a reservoir of Vibrio cholerae O1 in hydrographic basins of the state of Pernambuco, Brazil. The Scientific World Journal, v. 2013, p. 1-5, 2013.
dc.identifier1537-744X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23656
dc.identifier10.1155/2013/746254
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890630
dc.descriptionAfter the worldwide cholera epidemic in 1993, permanent environmental monitoring of hydrographic basins was established in Pernambuco, Brazil, where cholera is endemic. After a quiescent period, 4 rfbN (serogroup O1) positive water samples that were culture negative were detected by multiplex single-tube nested PCR (MSTNPCR); 2 of these were also ctxA (cholera toxin) positive. From May to June 2012, 30 V. cholerae O1 isolates were obtained by culturing samples. These isolates were analyzed for the presence of virulence genes by PCR, intergenic spacer region 16S-23S PCR (ISR-PCR), and pulsed field gel electrophoresis (PFGE). The isolates were positive for the rfbN gene and negative for the assessed pathogenic genes and were classified into 2 groups by ISR and the same profile by PFGE. Close genetic similarity was observed between them (2012) and environmental strains from 2004 to 2005, indicating the permanence of endemic V. cholerae O1 in the region.
dc.formatapplication/pdf
dc.languageeng
dc.publisherHindawi Publishing Corporation
dc.rightsopen access
dc.subjectDoenças endêmicas
dc.subjectPCR
dc.subjectCólera
dc.subjectEndemic Diseases
dc.subjectPCR
dc.subjectCholera
dc.subjectDNA Bacteriano
dc.subjectAnálise
dc.subjectReservatórios de Doenças
dc.subjectMicrobiologia
dc.subjectVibrio cholerae O1
dc.subjectIsolamento & purificação
dc.subjectMicrobiologia da Água
dc.subjectBrasil
dc.subjectProteínas de Bactérias
dc.subjectDNA Intergênico
dc.subjectReação em Cadeia da Polimerase Multiplex
dc.subjectMétodos
dc.subjectRNA Ribossômico 16S
dc.subjectRNA Ribossômico 23S
dc.subjectVibrio cholerae O1
dc.subjectClassificação
dc.subjectVibrio cholerae O1
dc.subjectGenética
dc.subjectFatores de Virulência
dc.titleThe aquatic environment as a reservoir of Vibrio cholerae O1 in hydrographic basins of the state of Pernambuco, Brazil
dc.typeArticle


Este ítem pertenece a la siguiente institución