Article
Proteomic analysis of the secretome of HepG2 cells indicates differential proteolytic processing after infection with dengue virus
Registro en:
CARUSO, Marjorlly B. et al. Proteomic analysis of the secretome of HepG2 cells indicates differential proteolytic processing after infection with dengue virus. Journal of Proteomics, v.151, p.106-113, July 2017.
1874-3919
10.1016/j.jprot.2016.07.011
Autor
Caruso, Marjolly B.
Trugilho, Monique R. O.
Higa, Luiza M.
Ferreira, André S. Teixeira
Perales, Jonas
Poian, Andrea T. Da
Zingali, Russolina B.
Resumen
Secretome analysis can be described as a subset of proteomics studies consisting in the analysis of the molecules secreted by cells or tissues. Dengue virus (DENV) infection can lead to a broad spectrum of clinical manifestations, with the severe forms of the disease characterized by hemostasis abnormalities and liver injury. The hepatocytes are a relevant site of viral replication and a major source of plasma proteins. Until now, we had limited information on the small molecules secreted by hepatic cells after infection by DENV. In the present study, we analysed a fraction of the secretome of mock- and DENV-infected hepatic cells (HepG2 cells) containing molecules with <10kDa, using different proteomic approaches. We identified 175 proteins, with 57 detected only in the samples from mock-infected cells, 59 only in samples from DENV-infected cells, and 59 in both conditions. Most of the peptides identified were derived from proteins larger than 10kDa, suggesting a proteolytic processing of the secreted molecules. Using in silico analysis, we predicted consistent differences between the proteolytic processing occurring in mock and DENV-infected samples, raising, for the first time, the hypothesis that differential proteolysis of secreted molecules would be involved in the pathogenesis of dengue. 2030-01-01