Article
Human enteric adenovirus F40/41 as a major cause of acute gastroenteritis in children in Brazil, 2018 to 2020
Registro en:
NASCIMENTO, Lilian Gonçalves do et al. Human enteric adenovirus F40/41 as a major cause of acute gastroenteritis in children in Brazil, 2018 to 2020. Scientific Reports, v. 12, 11220, p. 1 - 12, 2022.
2045-2322
10.1038/s41598-022-15413-1
Autor
Nascimento, Lilian Gonçalves do
Fialho, Alexandre Madi
Andrade, Juliana da Silva Ribeiro de
Assis, Rosane Maria Santos de
Fumian, Tulio Machado
Resumen
Human adenovirus (HAdV) types F40/41 have long been recognized as major viral agents of acute
gastroenteritis (AGE) in children. Despite this, studies on HAdV molecular epidemiology are sparse,
and their real impact is likely under-estimated. Thus, our goal was to investigate HAdV incidence,
enteric and non-enteric types circulation, co-detections with rotavirus and norovirus and DNA
shedding in stool samples from inpatients and outpatients from eleven Brazilian states. During the
three-year study, 1012 AGE stool samples were analysed by TaqMan-based qPCR, to detect and
quantify HAdV. Positive samples were genotyped by partial sequencing of the hexon gene followed
by phylogenetic analysis. Co-detections were accessed by screening for rotavirus and norovirus.
Overall, we detected HAdV in 24.5% of single-detected samples (n = 248), with a prevalence of
type F41 (35.8%). We observed a higher incidence in children between 6 to 24 months, without
marked seasonality. Additionally, we observed a statistically higher median viral load among singledetections
between enteric and non-enteric types and a significantly lower HAdV viral load compared
to rotavirus and norovirus in co-detections (p < 0.0001). Our study contributes to the knowledge of
HAdV epidemiology and reinforces the need for the inclusion of enteric types F40/41 in molecular
surveillance programs.