Article
Flow cytometric assay in peripheral blood of dogs--reference values for leukocytes from Brazilian beagles.
Registro en:
FUJIWARA, Ricardo Toshio et al. Flow cytometric assay in peripheral blood of dogs: reference values for leukocytes from Brazilian beagles. Pol. J. Vet. Sci. v. 8, n. 1, p. 17-22, 2005.
1505-1773
Autor
Fujiwara, Ricardo Toshio
Bueno, Lilian Lacerda
Vale, André Macedo
Silva, João Carlos França
Costa, Roberto Teodoro da
Quetz, Josiane da Silva
Coelho, George Luiz Lins Machado
Reis, Augusto Barbosa
Martins Filho, Olindo Assis
Genaro, Odair
Nascimento, Evaldo
Mayrink, Wilson
Resumen
Use of domestic reference values in the flow cytometry analysis is known to improve its accuracy by integrating local variations as gender, race and age. Up to date application of flow cytometry in veterinary medicine has been limited to describe the percentual values just for peripheral lymphocytes subsets of blood. We now report establishment of reference values for a wide range of proportional and absolute numbers of peripheral blood leukocytes, including T cells subsets, B cells, monocytes and eosinophils, applicable to the healthy population of Beagles in Brazil and other regions with similar demographic characteristics. Normal reference values were also established to estimate the gender-related differences. This information will provide clinical aid in the evaluation of immunologic status as well as standard values for experimental animals of dogs from Brazil and other similar regions