Article
Preliminary Characterization of Mitochondrial Genome of Melipona scutellaris, a Brazilian Stingless Bee
Registro en:
SILVERIO, Manuella Souza et al. Preliminary Characterization of Mitochondrial Genome of Melipona scutellaris, a Brazilian Stingless Bee. BioMed Research International, v. 2014, Article ID 927546, 6 pages
2314-6133
10.1155/2014/927546
Autor
Silverio, Manuella Souza
Rodovalho, Vinícius de Rezende
Bonetti, Ana Maria
Oliveira, Guilherme Corrêa de
Cuadros-Orellana, Sara
Vieira, Carlos Ueira
Santos, Anderson Rodrigues dos
Resumen
Bees are manufacturers of relevant economical products and have a pollinator role fundamental to ecosystems. Traditionally, studies focused on the genus Melipona have been mostly based on behavioral, and social organization and ecological aspects. Only recently the evolutionary history of this genus has been assessed using molecular markers, including mitochondrial genes. Even though these studies have shed light on the evolutionary history of the Melipona genus, a more accurate picture may emerge when full nuclear and mitochondrial genomes of Melipona species become available. Here we present the assembly, annotation, and characterization of a draft mitochondrial genome of the Brazilian stingless bee Melipona scutellaris usingMelipona bicolor as a reference organism. Using Illumina MiSeq data, we achieved the annotation of all protein coding genes, as well as the genes for the two ribosomal subunits (16S and 12S) and transfer RNA genes as well. Using the COI sequence as a DNA barcode, we found that M. cramptoni is the closest species to M. scutellaris.