Article
Aplysina (Porifera: Demospongiae) species identification through SSCP-ITS patterns
Registro en:
LAMARÃO, Flávia Rachel M. et al. Aplysina (Porifera: Demospongiae) species identification through SSCP-ITS patterns. Journal of the Marine Biological Association of the United Kingdom, v.90, n.4, p.845-850, 2010..
1469-7769
10.1017/S0025315409991202
Autor
Lamarão, Flávia Rachel M.
Reis, Estéfane C.
Simão, Tatiana A.
Albano, Rodolpho M.
Lôbo-Hajdu, Gisele
Resumen
Keratose sponges have no spicules and limited robust morphological characters, causing severe problems for taxonomic classification. Single-strand conformational polymorphism (SSCP) analysis of PCR amplified internal transcribed spacers (ITS) of nuclear ribosomal RNA (rRNA) genes was used to elucidate speciation patterns in marine sponges of the genus Aplysina. In
this study, Aplysina species with non-ambiguous traditional morphological alpha taxonomy were used to generate standard
SSCP-ITS patterns, which were compared with patterns from alleged new species. Our results show that ITS-SSCP patterns
allowed the differentiation of Aplysina sponges, demonstrating the advantage of a method that explores the secondary structure
compared to direct sequencing. 2030-01-01