Article
In situ encapsulation of rivastigmine in TAT-functionalized P(MMA-co-AA) nanoparticles through miniemulsion polymerization
Registro en:
MANGIA, L. H. R. et al. In situ encapsulation of rivastigmine in TAT-functionalized P(MMA-co-AA) nanoparticles through miniemulsion polymerization. Colloids and Surfaces A: Physicochemical and Engineering Aspects, v. 624, 126776, p. 1-11, May 2021.
0927-7757
10.1016/j.colsurfa.2021.126776
Autor
Mangia, L.H.R.
Ferraz, H. C.
Souza, R. S. D.
Pereira, M. C. S.
Pinto, J. C.
Resumen
The present study aimed to encapsulate rivastigmine (a drug used for treatment of the Alzheimer disease) in poly
(methyl methacrylate – co – acrylic acid), P(MMA-co-AA), nanoparticles functionalized with the transcription
activator peptide (TAT) for biomedical applications. Encapsulation was performed in situ through miniemulsion
polymerization at different preparation conditions and the highest encapsulation efficiencies (around 80%) were
obtained when the comonomer mixture contained 7.5 wt% of acrylic acid in methyl methacrylate. Afterwards,
functionalization trials of the previously prepared polymer nanoparticles were performed through chemical
adsorption, leading to high TAT immobilization efficiencies (94%). Additionally, toxicity bioassays conducted
with Caco-2 cells monolayers revealed that the cytotoxicity of obtained particles was low and did not depend on
the nanoparticle composition. Besides, the obtained results indicated that the functionalized nanoparticles could
cross the intestinal epithelial barrier successfully. Finally, the obtained particles presented good stability, could
be prepared with high encapsulation and functionalization efficiencies, and presented advantageous biological
properties that encourage the future development of biomedical uses. 2023