Characterization of main cytokine sources from the innate and adaptive immune responses following primary 17DD yellow fever vaccination in adults

dc.creatorSilva, Maria Luiza
dc.creatorMartins, Marina Angela
dc.creatorSanto, Luçandra Ramos Espírito
dc.creatorAzevedo, Ana Carolina Campi
dc.creatorLemos, Denise da Silveira
dc.creatorRibeiro, José Geraldo Leite
dc.creatorHomma, Akira
dc.creatorKroon, Erna Geessien
dc.creatorCarvalho, Andréa Teixeira de
dc.creatorSantos, Silvana Maria Elói
dc.creatorMartins Filho, Olindo Assis
dc.date2013-09-30T18:29:48Z
dc.date2013-09-30T18:29:48Z
dc.date2011
dc.date.accessioned2023-09-26T23:12:11Z
dc.date.available2023-09-26T23:12:11Z
dc.identifierSILVA, Maria Luiza et al. Characterization of main cytokine sources from the innate and adaptive immune responses following primary 17DD yellow fever vaccination in adults. Vaccine, v. 29, p. 583-592, 2011.
dc.identifier0264-410X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7020
dc.identifier10.1016/j.vaccine.2010.08.046
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887970
dc.descriptionThe mechanisms of immune response following yellow fever (YF-17DD) vaccination are still poorly understood. In this study, we have performed a longitudinal investigation (days 0, 7, 15 and 30) to characterize the cytokine profile of innate and adaptive immunity following YF-17DD first-time vaccination. Data from non-stimulated cultures demonstrated a prominent participation of the innate immunity with increased frequency of TNF-α(+) neutrophils and IFN-γ(+) NK-cells at day 7 besides TNF-α(+) monocytes at day 7, day 15 and day 30. Increased frequency of IL-10(+) monocytes was observed at day 15 and day 30, and decreased percentage of IL-4(+) NK-cells were detected at day 7, day 15 and day 30. Time-dependent and oscillating cytokine pattern was observed in CD4(+) T-cells, with low percentage of IL-12(+), IL-4(+) and IL-10(+) cells at day 7 and increased frequency of TNF-α(+) cells at day 15 besides IFN-γ(+) and IL-5(+) cells at day 15 and day 30. Later changes with increased percentage of IL-12(+) and IL-5(+)CD8(+) T-cells were observed at day 30. Increased frequency of IL-10(+) B-cells was observed at day 15, when seroconversion was detected in all vaccinees. The overall cytokine analysis of non-stimulated leukocytes showed a transient shift towards a pro-inflammatory profile at day 7, mainly due to changes in the innate immunity, which draws back toward a mixed/regulatory pattern at day 15 and day 30. The changes induced by the in vitro YF-17DD vaccine-stimulation were mainly observed at day 0 and day 7 (before seroconversion) with minor changes at day 15 and day 30 (after seroconversion). These data support the hypothesis that a complex network with mixed pro/anti-inflammatory cytokine profile is associated with the establishment of the protective immunity following YF-17DD primo-vaccination, free of adverse events.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Science
dc.rightsrestricted access
dc.subject17DD vaccine
dc.subjectYellow fever
dc.subjectCytokines
dc.subjectInnate and adaptive immunity
dc.titleCharacterization of main cytokine sources from the innate and adaptive immune responses following primary 17DD yellow fever vaccination in adults
dc.typeArticle


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