Ru(II)-Thymine Complex Causes Cell Growth Inhibition and Induction of Caspase-Mediated Apoptosis in Human Promyelocytic Leukemia HL-60 Cells

dc.creatorOliveira, Maiara de Souza
dc.creatorSantana, Ádila Angélica Dantas de
dc.creatorCorrea, Rodrigo S
dc.creatorSoares, Milena Botelho Pereira
dc.creatorBatista, Alzir Azevedo
dc.creatorBezerra, Daniel Pereira
dc.date2018-06-29T16:37:13Z
dc.date2018-06-29T16:37:13Z
dc.date2018
dc.date.accessioned2023-09-26T23:12:06Z
dc.date.available2023-09-26T23:12:06Z
dc.identifierOLIVEIRA, M. S. et al. Ru(II)-Thymine Complex Causes Cell Growth Inhibition and Induction of Caspase-Mediated Apoptosis in Human Promyelocytic Leukemia HL-60 Cells. International Journal of Molecular Sciences, v. 19, p. 1609, 2018.
dc.identifier1661-6596
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/27202
dc.identifier10.3390/ijms19061609
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887955
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). In particular, R.S.C. would like to thank CNPq for the grants #403588/2016-2 and #308370/2017-1.
dc.descriptionRuthenium-based compounds represent a class of potential antineoplastic drugs. Recently, we designed, synthesized, and identified the Ru(II)-thymine complex [Ru(PPh₃)₂(Thy)(bipy)]PF₆ (where PPh = triphenylphosphine, Thy = thymine and bipy = 2,2'-bipyridine) as a potent cytotoxic agent with the ability to bind to DNA and human and bovine serum albumins. In this study, the underlying cytotoxic mechanism of the [Ru(PPh₃)₂(Thy)(bipy)]PF₆ complex was assessed. This complex displayed potent cytotoxicity in different cancer cell lines; the morphology that is associated with apoptotic cell death, increased internucleosomal DNA fragmentation without cell membrane permeability, loss of the mitochondrial transmembrane potential, increased phosphatidylserine externalization, and caspase-3 activation were observed in human promyelocytic leukemia HL-60 cells that were treated with the complex. Moreover, pretreatment of HL-60 cells with Z-VAD(OMe)-FMK, a pan-caspase inhibitor, partially reduced the apoptosis that was induced by the complex, indicating that the apoptotic cell death occurred through a caspase-mediated pathway. In conclusion, the [Ru(PPh₃)₂(Thy)(bipy)]PF₆ complex displays potent cytotoxicity to different cancer cells and induces caspase-mediated apoptosis in HL-60 cells.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectRutênio
dc.subjectTimina
dc.subjectCitotoxicidade
dc.subjectApoptose
dc.subjectHL-60
dc.subjectRuthenium
dc.subjectThymine
dc.subjectCytotoxicity
dc.subjectApoptosis
dc.subjectHL-60
dc.titleRu(II)-Thymine Complex Causes Cell Growth Inhibition and Induction of Caspase-Mediated Apoptosis in Human Promyelocytic Leukemia HL-60 Cells
dc.typeArticle


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