A comparison of molecular methods for hepatitis B virus (HBV) DNA detection from oral fluid samples

dc.creatorPortilho, Moyra Machado
dc.creatorMartins, Patrícia Pais
dc.creatorLampe, Elisabeth
dc.creatorVillar, Livia Melo
dc.date2017-01-17T16:56:45Z
dc.date2017-01-17T16:56:45Z
dc.date2012
dc.date.accessioned2023-09-26T23:11:19Z
dc.date.available2023-09-26T23:11:19Z
dc.identifierPORTILHO, Moyra Machado; et al. A comparison of molecular methods for hepatitis B virus (HBV) DNA detection from oral fluid samples. Journal of Medical Microbiology, v.61, p. 844–851, 2012.
dc.identifier0022-2615
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/16874
dc.identifier10.1099/jmm.0.040238-0
dc.identifier1473-5644
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887809
dc.descriptionThe objective of the present study was to evaluate four commercial DNA extraction methods and three PCR protocols for hepatitis B virus (HBV) detection in artificially contaminated oral fluid samples. The extraction protocols were selected based on ease of use and cost, and were also compared with respect to sensitivity and cost. Prior PCR optimization was conducted, in which the sample volume for DNA extraction and the concentrations of DNA and Taq DNA polymerase in the PCR were adjusted. One-round PCR, used to amplify the core region of the HBV genome, achieved high levels of sensitivity in comparison with nested and semi-nested PCR experiments that were designed for the amplification of HBV surface protein genes. Of the four extraction protocols evaluated, the RTP DNA/RNA Virus Mini kit and the QIAamp DNA Mini kit gave the highest recovery rates, presenting 20 copies of HBV DNA ml(-1) as the limit of detection. These results suggest that HBV DNA can be detected from oral fluid samples but that the optimization of the PCR assays and the choice of extraction methods must be determined by laboratories before the implementation of this method in routine diagnostics.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherMicrobiology Society
dc.rightsrestricted access
dc.subjectVírus da Hepatite B
dc.subjectMétodo de extração de DNA
dc.subjectFluido oral
dc.subjectReação em Cadeia da Polimerase
dc.subjecthepatitis B virus (HBV)
dc.subjectPCR protocols
dc.subjectoral fluid
dc.subjectDNA extraction methods
dc.titleA comparison of molecular methods for hepatitis B virus (HBV) DNA detection from oral fluid samples
dc.typeArticle


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