Article
Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study
Registro en:
ANDRADE, Luciana O. et al. Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study. Mem Inst Oswaldo Cruz,v.105, n.6, p.834-837, Sept. 2010
0074-0276
1678-8060
Autor
Andrade, Luciana O.
Galvão, Lúcia M. C.
Meirelles, Maria de Nazareth S. L.
Chiari, Egler
Pena, Sergio D. J.
Macedo, Andrea M.
Resumen
We have previously demonstrated selection favoring the JG strain of Trypanosoma cruzi in hearts of BALB/c mice that were chronically infected with an equal mixture of the monoclonal JG strain and a clone of the Colombian strain, Col1.7G2. To evaluate whether cell invasion efficiency drives this selection, we infected primary cultures of BALB/c cardiomyocytes using these same T. cruzi populations. Contrary to expectation, Col1.7G2 parasites invaded heart cell cultures in higher numbers than JG parasites; however, intracellular multiplication of JG parasites was more efficient than that of Col1.7G2 parasites. This phenomenon was only observed for cardiomyocytes and not for cultured Vero cells. Double infections (Col1.7G2 + JG) showed similar results. Even though invasion might influence tissue selection, our data strongly suggest that intracellular development is important to determine parasite tissue tropism.