Article
Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats
Registro en:
BARROS, Juliana Helena da Silva et al. Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats. Pathogens, v. 9, n. 736, 12p, 2020.
2076-0817
10.3390/pathogens9090736
Autor
Barros, Juliana Helena da Silva
Roque, André Luiz Rodrigues
Xavier, Samanta Cristina das Chagas
Nascimento, Kátia Cristina Silva
Toma, Helena Keiko
Madeira, Maria de Fatima
Resumen
This study describes the morphological, biochemical, and molecular di erences among
Trypanosoma dionisii isolates from hemocultures of hematophagous (Desmodus rotundus; n = 2) and
insectivorous (Lonchorhina aurita; n = 1) bats from the Atlantic Rainforest of Rio de Janeiro, Brazil.
Fusiform epimastigotes from the hematophagous isolates were elongated, whereas those of the
insectivorous isolate were stumpy, reflected in statistically evident di erences in the cell body and
flagellum lengths. In the hemocultures, a higher percentage of trypomastigote forms (60%) was
observed in the hematophagous bat isolates than that in the isolate from the insectivorous bat (4%),
which demonstrated globular morphology. Three molecular DNA regions were analyzed: V7V8 (18S
rDNA), glycosomal glyceraldehyde 3-phosphate dehydrogenase gene, and mitochondrial cytochrome
b gene. The samples were also subjected to multilocus enzyme electrophoresis and random amplified
polymorphic DNA analysis. All isolates were identified as T. dionisii by phylogenetic analysis.
These sequences were clustered into two separate subgroups with high bootstrap values according
to the feeding habits of the bats from which the parasites were isolated. However, other T. dionisii
samples from bats with di erent feeding habits were found in the same branch. These results support
the separation of the three isolates into two subgroups, demonstrating that di erent subpopulations
of T. dionisii circulate among bats.