Article
Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
Registro en:
PAIVA, M. H. S. et al. Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies. Genetics and Molecular Biology, v. 40, n. 3, p. 597–599, 22 maio 2017.
1415-4757
10.1590/1678-4685-GMB-2016-0312
Autor
Paiva, Marcelo Henrique Santos
Guedes, Duschinka Ribeiro Duarte
Leal, Walter Soares
Ayres, Constância Flávia Junqueira
Resumen
Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).