Article
In silico identification of glycosylphosphatidylinositol-anchored proteins in Paracoccidioides spp
Registro en:
GONÇALES, Relber Aguiar et al. In silico identification of glycosylphosphatidylinositol-anchored proteins in Paracoccidioides spp. Future Microbiology, v. 16, n. 8, p. 589-606, 2021. do:10.2217/fmb-2020-0282
1746-0913
10.2217/fmb-2020-0282
Autor
Gonçales, Relber Aguiar
Salamanca, Ayda Lm
Rb Júnior, Luiz
Silva, Kleber Sf E
Vasconcelos, Elton Jr de
Reis, Thaila F Dos
Castro, Ricardo C
Ruy, Patrícia de C
Romagnoli, Bárbara
Ruiz, Jerônimo Conceição
Pereira, Maristela
Soares, Célia M de A
Coelho, Paulo Sr
Resumen
Aim: To predict glycosylphosphatidylinositol (GPI)-anchored proteins in the genome of Paracoccidioides brasiliensis and Paracoccidioides lutzii. Materials & methods: Five different bioinformatics tools were used for predicting GPI-anchored proteins; we considered as GPI-anchored proteins those detected by at least two in silico analysis methods. We also performed the proteomic analysis of P. brasiliensis cell wall by mass spectrometry. Results: Hundred GPI-anchored proteins were predicted in P. brasiliensis and P. lutzii genomes. A series of 57 proteins were classified in functional categories and 43 conserved proteins were reported with unknown functions. Four proteins identified by in silico analyses were also identified in the cell wall proteome. Conclusion: The data obtained in this study are important resources for future research of GPI-anchored proteins in Paracoccidioides spp. to identify targets for new diagnostic tools, drugs and immunological tests.