Article
Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests
Registro en:
COUTINHO, Mariana Loner et al. Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Journal of Immunoassay and Immunochemistry, v. 28, p. 279-288, 2007.
1532-1819
10.1080/15321810701454870
Autor
Coutinho, Mariana Loner
Vasconcellos, Flavia Aleixo
Fernandes, C. P. H.
Seyffert, Núbia
Seixas, Fabiana Kommling
Ko, Albert Icksang
Dellagostin, Odir Antonio
Aleixo, José Antonio Guimarães
Resumen
Fundação Oswaldo Cruz (09224-7), Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (477609/2004-0,301920/2006-0 and 504227/2006-0) and National Institutes of Health, USA (AI034431 and
TW00919). LipL32 is the major lipoprotein in the membrane of pathogenic leptospira. In this work, we report on the production of monoclonal antibodies (MAbs) against recombinant LipL32 (rLipL32) and on the evaluation of their potential for use as reagents in diagnostic tests for leptospirosis. The MAbs were all of the IgG(2b) isotype and reacted specifically with native LipL32 in pathogenic serovars only. MAbs reacted in the same region of the rLipL32 molecule and their affinity constant was between 5x10(7) M(-1) and 6x10(6) M(-1). These results suggest that although the MAbs cannot be used together, they are well suited for diagnostic tests of leptospirosis based on LipL32 detection.