Article
Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil
Registro en:
FAVALESSA, Olivia Cometti et al. Molecular typing and in vitro antifungal susceptibility of Cryptococcus spp from patients in Midwest Brazil. Journal of Infection in Developing Countries, v. 8, n. 8, p.1037-1043, 2014.
1972-2680
10.3855/jidc.4446
2036-6590
Autor
Favalessa, Olivia Cometti
Paula, Daphine Ariadne Jesus de
Dutra, Valéria
Nakazato, Luciano
Tadano, Tomoko
Lazera, Márcia dos Santos
Wanke, Bodo
Trilles, Luciana
Szeszs, Maria Walderez
Silva, Dayane
Hahn, Rosane Christine
Resumen
Introduction: Cryptococcosis is a systemic fungal infection that affects humans and animals, mainly due to Cryptococcus neoformans and Cryptococcus gattii. Following the epidemic of acquired immunodeficiency syndrome (AIDS), fungal infections by C. neoformans have become more common among immunocompromised patients. Cryptococcus gattii has primarily been isolated as a primary pathogen in healthy hosts and occurs endemically in northern and northeastern Brazil. We to perform genotypic characterization and determine the in vitro susceptibility profile to antifungal drugs of the Cryptococcus species complex isolated from HIV-positive and HIV-negative patients attended at university hospitals in Cuiabá, MT, in the Midwestern region of Brazil.
Methodology: Micromorphological features, chemotyping with canavanine-glycine-bromothymol blue (CGB) agar and genotyping by URA5-RFLP were used to identify the species. The antifungal drugs tested were amphotericin B, fluconazole, flucytosine, itraconazole and voriconazole. Minimum inhibitory concentrations (MICs) were determined according to the CLSI methodology M27-A3.
Results: Analysis of samples yelded C. neoformans AFLP1/VNI (17/27, 63.0%) and C. gattii AFLP6/VGII (10/27, 37.0%). The MICs ranges for the antifungal drugs were: amphotericin B (0.5-1 mg/L), fluconazole (1-16 mg/L), flucytosine (1-16 mg/L), itraconazole (0.25-0.12 mg/L) and voriconazole (0.06-0.5 mg/L). Isolates of C. neoformans AFLP1/VNI were predominant in patients with HIV/AIDS, and C. gattii VGII in HIV-negative patients. The genotypes identified were susceptible to the antifungal drugs tested.
Conclusion: It is worth emphasizing that AFLP6/VGII is a predominant genotype affecting HIV-negative individuals in Cuiabá. These findings serve as a guide concerning the molecular epidemiology of C. neoformans and C. gattii in the State of Mato Grosso.
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Biological activity of Cryptococcus neoformans and Cryptococcus gattii from clinical and environmental isolates
Barbosa Júnior, Antônio Márcio; Santos, Bruno Fernandes de Oliveira; Carvalho, Erick de Oliveira; Mélo, Dângelly Lins Figuerôa Martins de; Trindade, Rita de Cássia; Stoianoff, Maria Aparecida de Resende -
Native trees of the Northeast Argentine: Natural hosts of the Cryptococcus neoformans–Cryptococcus gattii species complex
Cattana, Maria Emilia; Sosa, Maria de Los Angeles; Fernández, Mariana Soledad; Rojas, Florencia Dinorah; Mangiaterra, Magdalena Leonilda; Giusiano, Gustavo Emilio (Asociacion Espanola Micología, 2013-06)Antecedentes: En Argentina la información sobre la epidemiología y la distribución ambiental de Cryptococcus es escasa.. Resistencia es una ciudad que limita con Brasil y Paraguay, donde este hongo es endémico. Esto apoya ... -
Recuperación de Cryptococcus neoformans y Cryptococcus gattii a partir de fuentes ambientales en Cúcuta, Norte de Santander y su asociación con aislados clínicos
Angarita, Asbleide Karina; Cardenas Sierra, Denny Miley; Parra Giraldo, Claudia Marcela; Diaz Carvajal, Claudia Yaneth; Escandón Hernandez, Patricia Luz