Article
In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
Registro en:
SOUZA, Elen Mello de et al. In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 7, p. 697-701, Nov. 2004.
0074-0276
10.1590/s0074-02762004000700006
1678-8060
Autor
Souza, Elen Mello de
Henriques-Pons, Andrea
Bailly, Christian
Lansiaux, Amelie
Araujo-Jorge, Tania C.
Soeiro, Maria de Nazaré C.
Resumen
Primary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.