Pcr-based diagnosis for chagas disease in Bolivian children living in a active transmission area: comparison with conventional serological and parasitological diagnosis

dc.creatorWincker, P.
dc.creatorTelleria, J
dc.creatorBosseno, M. F.
dc.creatorCardoso, M. A.
dc.creatorMarques, P.
dc.creatorYaksic, N.
dc.creatorAznar, C.
dc.creatorLiegeard, P.
dc.creatorHontebeyrie, M.
dc.creatorNoireau, F.
dc.creatorMorel, Carlos Medicis
dc.creatorBreniere, S. F.
dc.date2016-06-30T11:39:05Z
dc.date2016-07-07T12:22:10Z
dc.date2016-06-30T11:39:05Z
dc.date2016-07-07T12:22:10Z
dc.date1997
dc.date.accessioned2023-09-26T22:54:34Z
dc.date.available2023-09-26T22:54:34Z
dc.identifierWINCKER, P. et al. Pcr-based diagnosis for chagas disease in Bolivian children living in a active transmission area: comparison with conventional serological and parasitological diagnosis. Parasitology, v. 114, p. 367-373, 1997.
dc.identifier0031-1820
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/14699
dc.identifier10.1017/S0031182096008554
dc.identifier1469-8161
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8884632
dc.descriptionA large field study has been performed in the Cochabamba region of Bolivia with the aim of comparing the polymerase chain reaction (PCR) with other diagnostic methods for Chagas' disease. The amplification of Trypanosoma cruzi-specific kinetoplast DNA sequences in blood samples was compared with classical serological methods, specific IgM detection and direct parasite visualization for 268 school children in a single village where Chagas' disease transmission is active. Of 113 children positive by classical serology or buffy coat examination, 106 were detected by PCR (sensitivity: 93.8%). We did not observe any significant difference of PCR sensitivity between initial (IgM and/or buffy coat positive) and indeterminate stage (only IgG positive) patients. Among the remaining 155 children unconfirmed as chagasic (who were either only IgM positive, IgG-, IgM-, and buffy coat-negative) only 1 case was PCR positive. This case may be due to DNA contamination, or to a very recent infection not detected otherwise, or to specific immune depression. These results show that PCR is a very sensitive parasitological test for Chagas' disease in active transmission regions. The future follow-up of the possibly infected patients who were only IgM-positive should clarify the interest of PCR and IgM tests in the detection of starting infections.
dc.formatapplication/pdf
dc.languagepor
dc.publisherCambridge University Press
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectChagas`Disease
dc.subjectPCR diagnosis
dc.subjectSerological diagnosis
dc.subjectTrypanosoma cruzi
dc.subjectDoença de Chagas
dc.subjectReação em Cadeia da Polimerase
dc.subjectDiagnóstico
dc.subjectSorodiagnóstico
dc.titlePcr-based diagnosis for chagas disease in Bolivian children living in a active transmission area: comparison with conventional serological and parasitological diagnosis
dc.typeArticle


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