Article
Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
Registro en:
VILARDO, Mônica de Castro Brito et al. Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 5, p. 499-501, Aug. 2006.
0074-0206
10.1590/s0074-02762006000500003
1678-8060
Autor
Vilardo, Mônica de Castro Britto
Thomé, Jacqueline Darc da Silva
Esteves, Wagner Thadeu Cardoso
Filgueiras, Ana Luzia Lauria
Oliveira, Selma Soares de
Resumen
A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.