Article
An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
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FERNANDES, Octavio et al. An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 91, n. 3, p. 279-284, May/June 1996.
0074-0276
10.1590/S0074-02761996000300005
1678-8060
Author
Fernandes, Octávio
Bozza, Marcelo
Pascale, Juan M.
Miranda, Antonio B. de
Lopes, Ulisses G.
Degrave, Wim M.
Abstract
Sequence analysis of Leishmania (Viannia) kDNA minicircles and analysis of multiple sequence alignments of the conserved region (minirepeats) of five distinct minicircles from L. (V.) braziliensis species with corresponding sequences derived from other dermotropic leishmanias indicated the presence of a sub-genus specific sequence. An oligonucleotide bearing this sequence was designed and used as a molecular probe, being able to recognize solely the sub-genus Viannia species in hybridization experiments. A dendrogram reflecting the homologies among the minirepeat sequences was constructed. Sequence clustering was obtained corresponding to the traditional classification based on similarity of biochemical, biological and parasitological characteristics of these Leishmania species, distinguishing the Old World dermotropic leishmanias, the New World dermotropic leishmanias of the sub-genus Leishmania and of the sub-genus Viannia.
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