Article
Proteomic map of Trypanosoma cruzi CL Brener: the reference strain of the genome project
Registro en:
SODRÉ, Cátia Lacerda; et al. Proteomic map of Trypanosoma cruzi CL Brener: the reference strain of the genome project. Arch. Microbiol., v.191, p.177-184, 2009.
0302-8933
10.1007/s00203-008-0439-6
1432-072X
Autor
Sodré, Cátia Lacerda
Chapeaurouge, Alexander D.
Kalume, Dário Eluan
Lima, Leila de Mendonça
Perales, Jonas
Fernandes, Octavio
Resumen
In this work two-dimensional gel electrophoresis combined with mass spectrometry was carried out in order to start the construction of a map of soluble proteins from epimastigote form of Trypanosoma cruzi CL Brener. This strain is a hybrid organism derived from two genotypes, T. cruzi I and T. cruzi II and was chosen for genome sequencing. The two-dimensional gel electrophoresis showed that most of proteins focused at 4-7 pH range. The identification demonstrated that several proteins were in multiple isoforms, such as tubulin and heat shock proteins. Potential targets for development of chemotherapeutic agents like arginine kinase, an enzyme absent from mammalian tissues that is involved in the energy supply of the parasite, were also detected. 2030-01-01