Article
A new miracidia hatching device for diagnosing schistosomiasis
Registro en:
JURBERG, Arnon D. et al. A new miracidia hatching device for diagnosing schistosomiasis. Memórias do Instituto Oswaldo Cruz. v. 103, n.1, p. 112-114, 2008.
0074-0276
Autor
Jurberg, Arnon Dias
Oliveira, Áureo A de
Lenzi, Henrique Leonel
Coelho, Paulo Marcos Zach
Resumen
Acknowledgements to Anna Carolina Lima, from Instituto de Pesquisa René Rachou-Fiocruz, MG, for her assistance on biostatistics, Dr. Vania Dias, from Laboratório de Patologia, Instituto Oswaldo Cruz-Fiocruz, RJ, for schematic drawings of the hatching device, Mr. João de Araújo Dutra Filho, from DIRAC-Fiocruz, RJ, for manufacturing the hatching device, Ms. Vera de Paula Ribeiro, from Laboratório de Esquistossomose, Centro de Pesquisa René Rachou-FIOCRUZ, MG, and Dr. John Kusel, from the Glasgow University, for English review. It is still imperative to develop a parasitological technique highly sensitive for diagnosing schistosomiasis in epidemiological and individual surveys. A simple and cheap hatching device with a collecting container was manufactured and tested under experimental conditions. Twelve Kato-Katz slides were performed as golden standard for comparison. Quantitative results can be carried out by counting miracidia in a plate and parasite load can be calculated (miracidia/gram of feces). Statistically significant values were higher in the hatching test. More sensitive results, with statistical significance, were achieved using 1.5 g of feces (which corresponds to 36 Kato-Katz slides) than by using the Kato-Katz method. Advantages of this technique and its limitations are presented.