Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi

dc.creatorPicchi-Constante, Gisele Fernanda Assine
dc.creatorHiraiwa, Priscila Mazzocchi
dc.creatorMarek, Martin
dc.creatorRogerio, Vanessa Zulkievicz
dc.creatorGuerra-Slompo, Eloise Pavão
dc.creatorRomier, Christophe
dc.creatorZanchin, Nilson Ivo Tonin
dc.date2022-05-26T12:04:46Z
dc.date2022-05-26T12:04:46Z
dc.date2022
dc.date.accessioned2023-09-26T22:38:59Z
dc.date.available2023-09-26T22:38:59Z
dc.identifierPICCHI-CONSTANTE, Gisele Fernanda Assine et al. Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi. Star Protocols, v. 3, n. 101324, p. 1–26, 2022.
dc.identifier2666-1667
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/52921
dc.identifier10.1016/j.xpro.2022.101324
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881482
dc.descriptionThis protocol outlines a new genetic complementation strategy to investigate gene function in Trypanosoma cruzi, the parasite causing Chagas disease. We combine CRISPR-Cas9 technology with recombination of variants of the target gene containing the desired mutations that are resistant to Cas9-cleavage, which enables detailed investigation of protein function. This experimental strategy overcomes some of the limitations associated with gene knockouts in T. cruzi. For complete details on the use and execution of this protocol, please refer to Marek et al. (2021).
dc.formatapplication/pdf
dc.languagepor
dc.publisherCell Press
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectGene Expression
dc.subjectCRISPR-Associated Protein 9
dc.subjectProtocols
dc.subjectGene Editing
dc.subjectExpresión Génica
dc.subjectProteína 9 Asociada a CRISPR
dc.subjectEdición Génica
dc.subjectExpression des gènes
dc.subjectProtéine-9 associée à CRISPR
dc.subjectProtocoles
dc.subjectÉdition de gène
dc.subjectExpressão Gênica
dc.subjectProteína 9 Associada à CRISPR
dc.subjectProtocolos
dc.subjectEdição de Genes
dc.titleEfficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi
dc.typeArticle


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