Detection of TH1/TH2 cytokine signatures in yellow fever 17DD first-time vaccinees through ELISpot assay

dc.creatorSantos, A. P.
dc.creatorMatos, D. C. S.
dc.creatorBertho, A. L.
dc.creatorMendonça, S. C. F.
dc.creatorMarcovistz, R.
dc.date2019-03-08T20:14:54Z
dc.date2019-03-08T20:14:54Z
dc.date2008
dc.date.accessioned2023-09-26T22:37:20Z
dc.date.available2023-09-26T22:37:20Z
dc.identifierSANTOS, A. P. et al. Detection of TH1/TH2 cytokine signatures in yellow fever 17DD first-time vaccinees through ELISpot assay. Cytokine, v.42, n.2, p.152-155, May 2008.
dc.identifier1043-4666
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32015
dc.identifier10.1016/j.cyto.2008.02.007
dc.identifier1096-0023
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8881128
dc.descriptionImmunity to yellow fever (YF) is conferred by the interplay of humoral and cellular immune response. Despite the extensive literature on the humoral immune response to the YF vaccine virus, little is known about its cellular immune response to vaccination. The analysis of cytokine production by ex-vivo antigen-stimulated T cells has been considered as a valuable tool for understanding cellular immune response. Thus, we have analyzed two TH1/TH2 signature cytokines (IFN-γ and IL-4) from 12 healthy first-time adults vaccinated with YF17DD virus. The cells, harvested on day 0 (before vaccination) and 7, 15 and 30 days after immunization were antigen-stimulated and analyzed by ELISpot. A significant increase in the number of spot-forming cells during the response to YF 17DD live virus stimulation by ELISpot assay was observed. IFN-γ-and IL-4-producing cells were significantly increased on the 15th day after vaccination in all volunteers. These results presented herein are important for understanding the role of cytokines in the immune response to YF 17DD virus.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier 12 Months
dc.rightsrestricted access
dc.subjectVacinação contra febre amarela
dc.subjectCitocinas
dc.subjectResposta imune da febre amarela
dc.subjectEnsayo de Immunospot Ligado a Enzimas
dc.subjectYellow fever vaccination
dc.subjectCytokines
dc.subjectELISpot
dc.subjectYellow fever immune response
dc.subjectEnsayo de Immunospot Ligado a Enzimas
dc.titleDetection of TH1/TH2 cytokine signatures in yellow fever 17DD first-time vaccinees through ELISpot assay
dc.typeArticle


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