Article
Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
Registro en:
PINTO, Patrícia M. et al. Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 2, p. 147-152, Mar. 2004.
0074-0276
10.1590/S0074-02762004000200006
1678-8060
Autor
Pinto, Patrícia M.
Resende, Maria A.
Koga-Ito, Cristiane Y.
Tendler, Miriam
Resumen
The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from
different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis
using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are
efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in
providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid
identification of pathogenic fungi.