Article
Immunodetection of toxins in historesin-embedded sections of Phoneutria nigriventer venom glands using laser confocal scanning microscopy
Registro en:
SILVA, Luciana Maria et al. Immunodetection of toxins in historesin-embedded sections of Phoneutria nigriventer venom glands using laser confocal scanning microscopy. Toxicon, v. 167, p. 168-171, 2019.
0041-0101
10.1016/j.toxicon.2019.06.018
Autor
Silva, Luciana Maria
Pimenta, Rafael Nacif
Ortolani, Paula Ladeira
Moura, Layla Mosqueira
Cordeiro, Marta do Nascimento
Chavez-Olórtegui, Carlos
Pimenta, Paulo Filemon Paolucci
Fortes-Dias, Consuelo Latorre
Resumen
In the last decades, main advances were achieved in the identification, structural and pharmacological characterization of Phoneutria nigriventer toxins. However, studies on the venom-producing apparatus are rare. Presently, we applied immunolabeling to historesin-embedded cross-sections of P. nigriventer venom glands. Toxins and toxin-secreting cells were successfully located in situ, using laser confocal scanning microscopy. The methodological strategy was successful and may be applied in future studies on venom glands and other secreting tissues, in general. 2022-01-01