Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorNovelli, V. M.
dc.creatorCristofani, M.
dc.creatorMachado, M. A.
dc.date2014-05-27T11:20:11Z
dc.date2016-10-25T18:16:46Z
dc.date2014-05-27T11:20:11Z
dc.date2016-10-25T18:16:46Z
dc.date2000-12-01
dc.date.accessioned2017-04-06T00:58:28Z
dc.date.available2017-04-06T00:58:28Z
dc.identifierActa Horticulturae, v. 535, p. 47-50.
dc.identifier0567-7572
dc.identifierhttp://hdl.handle.net/11449/66372
dc.identifierhttp://acervodigital.unesp.br/handle/11449/66372
dc.identifierWOS:000166400700004
dc.identifier2-s2.0-0037646174
dc.identifierhttp://www.actahort.org/books/535/535_4.htm
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/887952
dc.descriptionSweet orange is considered a very important species in the citrus world market and presents wide morphological variability. However, its characterization at the molecular level by random amplified polymorphic DNA (RAPD) and isozyme markers is not appropriate. Microsatellite or simple sequence repeats (SSRs) have been suggested as ideal for studies in cultures of vegetative propagation and as value markers for mapping in several species. However, information on microsatellite polymorphism in citrus species is scarce. In this work, microsatellite markers (AG-repeats) were developed from an enrichment library of genomic DNA of sweet orange cv. Pera (Citrus sinensis [L.] Osbeck), and 31 cultivars of sweet orange were evaluated. Evaluation of 18 microsatellite primers did not permit differentiation of the varieties studied. New microsatellite primers are being evaluated with the aim of detecting polymorphisms among the cultivars and closely related species to be used in genetic mapping programs.
dc.languageeng
dc.relationActa Horticulturae
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBreeding
dc.subjectMolecular markers
dc.subjectPolymerase chain reaction
dc.subjectPolymorphism
dc.titleEvaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck)
dc.typeOtro


Este ítem pertenece a la siguiente institución