Article
Polyketide Synthase Gene Diversity within the Microbiome of the Sponge Arenosclera brasiliensis, Endemic to the Southern Atlantic Ocean
Registro en:
SILVA, Amaro E. Trindade; et al. Polyketide Synthase Gene Diversity within the Microbiome of the Sponge Arenosclera brasiliensis, Endemic to the Southern Atlantic Ocean. Applied and Environmental Microbiology, v.79, n.5, p.1598-1605, Mar. 2013.
10.1128/AEM.03354-12
1098-5336
Autor
Silva, Amaro E. Trindade
Rua, Cintia P. J.
Andrade, Bruno G. N.
Vicente, Ana Carolina Paulo
Silva, Genivaldo G. Z.
Berlinck, Roberto G. S.
Thompson, Fabiano L.
Resumen
Microbes associated with marine sponges are considered important producers of bioactive, structurally unique polyketides. The
synthesis of such secondary metabolites involves type I polyketide synthases (PKSs), which are enzymes that reach a maximum
complexity degree in bacteria. The Haplosclerida sponge Arenosclera brasiliensis hosts a complex microbiota and is the source of
arenosclerins, alkaloids with cytotoxic and antibacterial activity. In the present investigation, we performed high-throughput
sequencing of the ketosynthase (KS) amplicon to investigate the diversity of PKS genes present in the metagenome of A. brasiliensis.
Almost 4,000 ketosynthase reads were recovered, with about 90% annotated automatically as bacterial. A total of 235 bacterial
KS contigs was rigorously assembled from this sequence pool and submitted to phylogenetic analysis. A great diversity of
six type I PKS groups has been consistently detected in our phylogenetic reconstructions, including a novel and A. brasiliensisexclusive
group. Our study is the first to reveal the diversity of type I PKS genes in A. brasiliensis as well as the potential of its
microbiome to serve as a source of new polyketides.