Article
Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies
Registro en:
BRAZIL, Beatriz Gomes; LINO-NETO, José; BRAZIL, Reginaldo Peçanha. Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, n. 2, p. 287-290, 2003.
0074-0276
10.1590/S0074-02762003000200021
Autor
Brazil, Beatriz Gomes
Neto, José Lino
Brazil, Reginaldo Peçanha
Resumen
Phlebotominae sand fly specimens were prepared for histological and physiological studies. Different fixatives were tested on sectioned and whole bodied adult females in order to obtain good fixation and provide satisfactory penetration of the embedding media. All fixed specimens were infiltrated (up to seven days under 5ºC) and embedded in hydroxyethyl metacrylate. Two-three µm sections were stained, mounted in Canada balsam and observed by light microscopy. Best results were achieved when whole bodied insects were double fixed in Bouin's and Carnoy's fluids (4 h/2 h) and stained in Hematoxilin/Eosin or fixed in calcium formaldehyde and stained in mercury bromophenol blue.